Influence of plasma on the physical properties of the ointments with quercetin

Effects of two independent variables – the content of quercetin (0 or 1 or 1.5 or 5 %) and the content of plasma (0 or 2 or 4 or 6 %) – on the organoleptic properties and rheological parameters of model formulations prepared on an amphiphilic base were estimated. The consistency of all ointments was uniform, and the content of quercetin and plasma lay within the predefined range. Tested ointments are non-Newtonian systems. The content of quercetin and plasma was found to have a significant effect on the rheological properties of the ointments. An increase in the content of plasma in ointments was accompanied by a significant increase in their hardness, viscosity and shear stress and a reduction of their spreadability. The best rheological properties were shown by formulation F-3, containing 1.5 % of quercetin and 2 % of plasma

Rocznik Lubuski (t.32, cz.1): Dziedzictwo kulturowe Środkowego Nadodrza

Pod redakcją: Wojciecha Eckerta

Rocznik Lubuski (t. 31, cz.1): Muzea i ochrona dziedzictwa kulturowego na Środkowym Nadodrzu

Muzea i Ochrona Dziedzictwa Kulturowego na Środkowym Nadodrzu

The Influence of Strontium on Bone Tissue Metabolism and Its Application in Osteoporosis Treatment

Osteoporosis is a chronic disease characterized by low bone mass caused by increased bone turnover and impaired bone microarchitecture. In treatment, we use antiresorptive or anabolic drugs, which usually have a unidirectional effect, i.e., they inhibit the activity of osteoclasts or stimulate the effect of osteoblasts. Strontium ranelate is an anti-osteoporosis drug with a unique mechanism of action (used primarily in postmenopausal women). Unlike other medicines, it has a multidirectional effect on bone tissue, intensifying osteoblastogenesis while inhibiting osteoclastogenesis. It turns out that this effect is demonstrated by strontium ions, an element showing physical and chemical similarity to calcium, the basic element that builds the mineral fraction of bone. As a result, strontium acts through the calcium-sensing receptor (CaSR) receptor in bone tissue cells. In recent years, there has been a significant increase in interest in the introduction of strontium ions in place of calcium ions in ceramics used as bone replacement materials for the treatment of bone fractures and defects caused by osteoporosis. The aim of this study was to summarize current knowledge about the role of strontium in the treatment of osteoporosis, its effects (in various forms), and the ways in which it is administered

Study of the role of oxidative stress in the synergistic action of curcumin and etoposide

Przedmiotem niniejszej pracy było zbadanie roli stresu oksydacyjnego w synergistycznym działaniu polifenolu kurkuminy i inhibitora topoizomerazy II — etopozydu w ludzkich białaczkowych promielocytach linii HL-60. Porównano także cytotoksyczne działanie tego polifenolu w komórkach białaczkowych i w prawidłowych, macierzystych komórkach hematopoetycznych z ekspresją receptora CD34. Stosowano 24-godzinne inkubacje komórek z kurkuminą (w stężeniu 20 μM) i/lub etopozydem (w stężeniu 10 μM). W celu określenia roli stresu oksydacyjnego w działaniu badanych związków zastosowano koinkubację z antyoksydantem (N-acetocysteiną) oraz preinkubowano komórki z prooksydantem, inhibitorem butioninosulfoksyiminą — BSO. Żywotność komórek oraz poziom wolnych rodników badano metodą cytometrii przepływowej, natomiast poziom glutationu analizowano metodą spektrofotometryczną. Wykazano, iż kurkumina nasila przeciwbiałaczkowe działanie etopozydu w komórkach HL-60 poprzez wpływ na poziom stresu oksydacyjnego. Potwierdza to korelujące ze wzrostem procentowego udziału komórek martwych zwiększenie poziomu wolnych rodników po zastosowaniu kombinacji kurkuminy i etopozydu, w porównaniu do grupy kontrolnej. Zastosowanie N-acetylocysteiny powodowało spadek liczy komórek martwych i poziomu wolnych rodników w komórkach inkubowanych z kombinacją kurkuminy i etopozydu. Przeciwny efekt zaobserwowano stosując preinkubację z BSO, a następnie inkubację z analizowanymi związkami. Poziom glutationu był niższy w grupach poddanych działaniu kurkuminy i/lub etopozydu w stosunku do kontroli. Preinkubacja z BSO prowadziła do dalszego spadku poziomu glutationu. Badany polifenol nie wywierał znamiennego cytotoksycznego działania w zdrowych komórkach hematopoetycznych CD34+ w stężeniu 20 μM, które znacząco obniżało żywotność komórek białaczkowych.Uzyskane wyniki wskazują, iż kurkumina może znaleźć zastosowanie w terapii addytywnej białaczek jako związek działający selektywnie w komórkach nowotworowych i nie wywierający cytotoksycznego wpływu w prawidłowych, proliferujących komórkach hematopoetycznych.The aim of the study was to evaluate the role of oxidative stress in synergistic action of polyphenol curcumin and topoisomerase-II inhibitor — etoposide in human promyelocytic leukemia cell line HL-60. The cytotoxic activity of the polyphenol was also compared in normal, hematopoietic stem cells with CD34 receptor expression and leukemia cells. Cells were incubated with curcumin (at a concentration of 20 μM) and/or etoposide (at a concentration of 10 μM) for 24-hours. To evaluate the role of oxidative stress in action of tested compounds cells were coincubated with antioxidant (N-acetylcysteine) and preincubated with pro-oxidant - buthionine sulfoximine (BSO). Viability of the cells and the level of free radicals was examined by flow cytometry, the level of glutathione was analyzed spectrophotometrically. It was shown, that curcumin enhances the antileukemic activity of etoposide through the influence on oxidative stress. This is confirmed by increase in the percentage of dead cells in correlation with an increase in the free radicals level, in comparison to the control group. Application of N-acetylcysteine resulted in a decrease in dead cells count and free radicals level in cells coincubated with the combination of curcumin and etoposide. The opposite effect was observed by pre-incubation with BSO, and then incubation with analyzed compounds. Glutathione levels were lower in the groups treated with curcumin and / or etoposide in comparison to the control. Preincubation with BSO led to a further decline in the level of glutathione. Tested polyphenol did not have a significant cytotoxic effects in healthy CD34+ hematopoietic cells at a concentration of 20 μM, which significantly decreased the viability of the leukemic cells.Obtained results indicate, that curcumin can be used in the treatment of leukemias as an additive, acting selectively in tumor cells and exerting no cytotoxic action in normal, proliferating hematopoietic cells

Biologically Inspired Collagen/Apatite Composite Biomaterials for Potential Use in Bone Tissue Regeneration—A Review

Type I collagen and nanocrystalline-substituted hydroxyapatite are the major components of a natural composite—bone tissue. Both of these materials also play a significant role in orthopedic surgery and implantology; however, their separate uses are limited; apatite is quite fragile, while collagen’s mechanical strength is very poor. Therefore, in biomaterial engineering, a combination of collagen and hydroxyapatite is used, which provides good mechanical properties with high biocompatibility and osteoinduction. In addition, the porous structure of the composites enables their use not only as bone defect fillers, but also as a drug release system providing controlled release of drugs directly to the bone. This feature makes biomimetic collagen–apatite composites a subject of research in many scientific centers. The review focuses on summarizing studies on biological activity, tested in vitro and in vivo

Influence of plasma on the physical properties of ointments with quercetin

Effects of two independent variables - the content of quercetin (0 or 1 or 1.5 or 5 %) and the content of plasma (0 or 2 or 4 or 6 %) - on the organoleptic properties and rheological parameters of model formulations prepared on an amphiphilic base were estimated. The consistency of all ointments was uniform, and the content of quercetin and plasma lay within the predefined range. Tested ointments are non-Newtonian systems. The content of quercetin and plasma was found to have a significant effect on the rheological properties of the ointments. An increase in the content of plasma in ointments was accompanied by a significant increase in their hardness, viscosity and shear stress and a reduction of their spreadability. The best rheological properties were shown by formulation F-3, containing 1.5 % of quercetin and 2 % of plasma